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BC

Research Assistant II

Boston Children's Hospital
  • 🇺🇸 United States
  • On-site
  • 1 day ago
  • $49,275.20 – $78,832.00 / year
  • calibration
  • Mac

Not enough detail in this posting to match

Job Posting Description

Position Summary
Join our flow core team and provide high-quality flow cytometry and cell sorting services to Boston Children's investigators and the wider Boston research community. This is a full-time position with full Boston Children's Hospital benefits and a 2-year minimum commitment.

Visit the Flow Cytometry webpage at Flow Cytometry | Boston Children's Research

Key Responsibilities
  • Perform fluorescence-activated cell sorting (FACS), including aseptic sorting of human and mouse samples, and monitor sort purity and recovery
  • Perform daily quality control, laser alignment, and calibration (e.g., with standardized beads), plus maintenance and troubleshooting, on our high-speed cell sorters and benchtop analyzers
  • Advise investigators on experimental design, including multicolor panel design, compensation, and controls (e.g., FMO, viability dye)
  • Analyze flow cytometry data (e.g., FlowJo) and assess data quality, and help investigators with gating strategy and presentation of results
  • Train new researchers and staff with instrument operation and good flow cytometry practice, and provide ongoing support
  • Handle billing, invoicing, and day-to-day lab operations
  • Stay current in the field by attending vendor and technical training, participating in journal club, and giving presentations on flow cytometry methods and new technologies.
Minimum Qualifications
Education: 
  • Bachelor's degree in biological or physical sciences (or related field) plus 2+ years of laboratory and flow cytometry experience in a clinical, industry, or academic setting, or a master degree in a related field
Experience: 
  • ​​​​​​​Hands-on experience with multicolor flow cytometry; cell sorting experience preferred
  • Working knowledge of biosafety practices for handling human-derived samples (BSL-2)
  • Ability to work independently, with strong problem-solving, attention to detail, and written and verbal communication
  • Proficiency with PC and Mac platforms and Microsoft Office
  • Comfortably working in a diverse, multicultural research environment and is a team player
  • Ability to safely lift 50 lbs. (with or without accommodation)
  • Status: Full-Time
  • Regular, Temporary, Per Diem: Regular
  • Standard Hours per Week: 40
  • Pay Range: $49275.20-$78832.00 Annual
  • Job Posting Category: Research
  • Remote Eligibility: Onsite Only

Research Assistant II · Boston Children's Hospital

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