BC
Research Assistant II
Boston Children's Hospital
- 🇺🇸 United States
- On-site
- 1 day ago
- $49,275.20 – $78,832.00 / year
- calibration
- Mac
1 day ago
Not enough detail in this posting to match
Job Posting Description
Position SummaryJoin our flow core team and provide high-quality flow cytometry and cell sorting services to Boston Children's investigators and the wider Boston research community. This is a full-time position with full Boston Children's Hospital benefits and a 2-year minimum commitment.
Visit the Flow Cytometry webpage at Flow Cytometry | Boston Children's Research
Key Responsibilities
- Perform fluorescence-activated cell sorting (FACS), including aseptic sorting of human and mouse samples, and monitor sort purity and recovery
- Perform daily quality control, laser alignment, and calibration (e.g., with standardized beads), plus maintenance and troubleshooting, on our high-speed cell sorters and benchtop analyzers
- Advise investigators on experimental design, including multicolor panel design, compensation, and controls (e.g., FMO, viability dye)
- Analyze flow cytometry data (e.g., FlowJo) and assess data quality, and help investigators with gating strategy and presentation of results
- Train new researchers and staff with instrument operation and good flow cytometry practice, and provide ongoing support
- Handle billing, invoicing, and day-to-day lab operations
- Stay current in the field by attending vendor and technical training, participating in journal club, and giving presentations on flow cytometry methods and new technologies.
Education:
- Bachelor's degree in biological or physical sciences (or related field) plus 2+ years of laboratory and flow cytometry experience in a clinical, industry, or academic setting, or a master degree in a related field
- Hands-on experience with multicolor flow cytometry; cell sorting experience preferred
- Working knowledge of biosafety practices for handling human-derived samples (BSL-2)
- Ability to work independently, with strong problem-solving, attention to detail, and written and verbal communication
- Proficiency with PC and Mac platforms and Microsoft Office
- Comfortably working in a diverse, multicultural research environment and is a team player
- Ability to safely lift 50 lbs. (with or without accommodation)
- Status: Full-Time
- Regular, Temporary, Per Diem: Regular
- Standard Hours per Week: 40
- Pay Range: $49275.20-$78832.00 Annual
- Job Posting Category: Research
- Remote Eligibility: Onsite Only
Research Assistant II · Boston Children's Hospital